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Multiple reference databases

Status: Open.

#2195 In benjjneb/dada2;

· owen-hughes42 opened on Apr 8, 2026

SILVA database v138.1

Status: Open.

#2194 In benjjneb/dada2;

· murheisa opened on Apr 7, 2026

Issue when mergerPairs

Status: Open.

#2193 In benjjneb/dada2;

· Channen1221 opened on Apr 3, 2026

V3-V4: huge chimeric read loss (more than 70% sequence variants flagged as chimeras)

Status: Open.

#2186 In benjjneb/dada2;

· pdhrati02 opened on Mar 25, 2026

Shortening truncLen increases chimeras and singletons (V4, MiSeq i100)

Status: Open.

#2185 In benjjneb/dada2;

· erhandler opened on Mar 24, 2026

Issues with qiime2-dada chimera removal

Status: Open.

#2184 In benjjneb/dada2;

· Salustinho opened on Mar 18, 2026

Metabarcoding data analysis of the V3–V4 region using the MiniSeq platform.

Status: Open.

#2183 In benjjneb/dada2;

· Paul-Fernandez-C opened on Mar 11, 2026

PacBio full-length 16S CCS data: almost all reads unique during dereplication and very few reads retained after denoising

Status: Open.

#2182 In benjjneb/dada2;

· alitcd opened on Mar 6, 2026

Low number of ASVs assigned to phylum

Status: Open.

#2181 In benjjneb/dada2;

· murheisa opened on Mar 3, 2026

How to choose truncation length from plotQualityProfile() for 16S V3–V4 (341F/805R) MiSeq i100 data?

Status: Open.

#2180 In benjjneb/dada2;

· biohp90 opened on Feb 24, 2026

Visualization of read quality profiles plot - thin heatmap

Status: Open.

#2178 In benjjneb/dada2;

· mmladinic opened on Feb 11, 2026

Mismatched R1/R2 PAIRS FOUND (these will crash filterAndTrim)

Status: Open.

#2177 In benjjneb/dada2;

· Kumaratenna opened on Feb 5, 2026