AfterQC is constantly counting wrong the total bases in paired end Miseq fastq reads. For example two paired end files with total bases 611,060,153 (as calculated by various programs) it seems to have only 582,146,000 total bases in AfterQC.
I was wondering why this difference exists, and if it affects the downstream filtering process.
AfterQC is constantly counting wrong the total bases in paired end Miseq fastq reads. For example two paired end files with total bases 611,060,153 (as calculated by various programs) it seems to have only 582,146,000 total bases in AfterQC.
I was wondering why this difference exists, and if it affects the downstream filtering process.